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fluorchemq multiimage iii  (Alpha Innotech)

 
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    Alpha Innotech fluorchemq multiimage iii
    Fluorchemq Multiimage Iii, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorchemq multiimage iii/product/Alpha Innotech
    Average 90 stars, based on 1 article reviews
    fluorchemq multiimage iii - by Bioz Stars, 2026-03
    90/100 stars

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    Western blot validation of host protein regulation. A , HeLa cells were mock-infected or infected for 24 h, or B , for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% ( A ), or in 4-16% gradient ( B ) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech <t>FluorChemQ</t> <t>MultiImage</t> <t>III</t> instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A . *: not detected in indicated fraction; †: based on single peptide only.
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    Western blot validation of host protein regulation. A , HeLa cells were mock-infected or infected for 24 h, or B , for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% ( A ), or in 4-16% gradient ( B ) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech <t>FluorChemQ</t> <t>MultiImage</t> <t>III</t> instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A . *: not detected in indicated fraction; †: based on single peptide only.
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    Western blot validation of host protein regulation. A , HeLa cells were mock-infected or infected for 24 h, or B , for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% ( A ), or in 4-16% gradient ( B ) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech FluorChemQ MultiImage III instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A . *: not detected in indicated fraction; †: based on single peptide only.

    Journal: Virology Journal

    Article Title: HeLa cell response proteome alterations induced by mammalian reovirus T3D infection

    doi: 10.1186/1743-422X-10-202

    Figure Lengend Snippet: Western blot validation of host protein regulation. A , HeLa cells were mock-infected or infected for 24 h, or B , for indicated periods of time, harvested and lysed with 0.5% NP-40 detergent. The cytosolic and nuclear fractions were separately purified, dissolved in SDS electrophoresis sample buffer, and proteins resolved in 10% ( A ), or in 4-16% gradient ( B ) SDS-PAGE, transferred to PVDF, and probed with indicated antibodies. Antibody binding was detected with HRP-conjugated secondary antibodies and ECL, and visualized with an Alpha Innotech FluorChemQ MultiImage III instrument. Molecular weight standards are indicated at left and SILAC-measured ratios are indicated on right in A . *: not detected in indicated fraction; †: based on single peptide only.

    Article Snippet: Bands were developed by enhanced chemiluminescence and imaged with an Alpha Innotech FluorChemQ MultiImage III instrument.

    Techniques: Western Blot, Infection, Purification, Electrophoresis, SDS Page, Binding Assay, Molecular Weight